AVALIAÇÃO DE BIOMARCADORES NO SANGUE PERIFÉRICO POR CITOMETRIA DE FLUXO COMO MEIO DE ORIENTAÇÃO PARA A IMUNOTERAPIA EM TUMORES SÓLIDOS: REVISÃO DA LITERATURA

  • Ana Catrina Trigo Immunology Department, Portuguese Institute of Oncology of Porto, Porto, Portugal; Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, Porto, Portugal; Experimental Pathology and Therapeutics Group, Portuguese Institute of Oncology, Porto, Portugal http://orcid.org/0000-0002-0383-7266
  • Patrícia Maia Immunology Department, Portuguese Institute of Oncology of Porto, Porto, Portugal; Department of Chemistry, University of Aveiro, Aveiro, Portugal; Experimental Pathology and Therapeutics Group, Portuguese Institute of Oncology, Porto, Portugal http://orcid.org/0000-0003-1216-3280
  • Inês Godinho Immunology Department, Portuguese Institute of Oncology of Porto, Porto, Portugal http://orcid.org/0000-0001-7952-8702
  • Catarina A. Rodrigues Experimental Pathology and Therapeutics Group, Portuguese Institute of Oncology, 4200-162 Porto, Portugal; Faculty of Medicine of the University of Porto (FMUP), Porto, Portugal http://orcid.org/0000-0002-1305-8055
  • Maria Emília Sousa Immunology Department, Portuguese Institute of Oncology of Porto, Porto, Portugal http://orcid.org/0000-0002-4965-9530
  • Ana Marta Pires Immunology Department, Portuguese Institute of Oncology of Porto, Porto, Portugal http://orcid.org/0000-0002-9011-5818
  • Carla Azevedo Immunology Department, Portuguese Institute of Oncology of Porto, Porto, Portugal http://orcid.org/0000-0002-6360-2100
  • Lúcio Lara Santos Experimental Pathology and Therapeutics Group, Portuguese Institute of Oncology, Porto, Portugal; Health Science Faculty, University of Fernando Pessoa, Porto, Portugal; Department of Surgical Oncology, Portuguese Institute of Oncology of Porto, Porto, Portugal; Porto Comprehensive Cancer Centre (P.ccc), Porto, Portugal http://orcid.org/0000-0002-0521-5655
  • Carlos Palmeira Immunology Department, Portuguese Institute of Oncology of Porto, Porto, Portugal; Experimental Pathology and Therapeutics Group, Portuguese Institute of Oncology, Porto, Portugal; Health Science Faculty, University of Fernando Pessoa, Porto, Portugal; Porto Comprehensive Cancer Centre (P.ccc), Porto, Portugal http://orcid.org/0000-0002-4833-2202
  • Gabriela Martins Immunology Department, Portuguese Institute of Oncology of Porto, Porto, Portugal; Experimental Pathology and Therapeutics Group, Portuguese Institute of Oncology, Porto, Portugal; Porto Comprehensive Cancer Centre (P.ccc), Porto, Portugal http://orcid.org/0000-0002-1367-8852

Resumo

O tratamento do cancro é uma área em intensa e permanente atualização, nomeadamente com a descoberta da imunoterapia como modalidade terapêutica clinicamente eficaz para diversos tipos de cancro. Entre estas terapias inovadoras, as terapias com inibidores de checkpoints imunológicos (ICI) têm demonstrado apresentar respostas significativas e duradouras. No entanto, esta resposta não ocorre para todos os doentes, ou seja, alguns pacientes não beneficiam desta terapia. Devido a esta heterogeneidade na resposta à imunoterapia, persiste a necessidade urgente de identificar e estabelecer biomarcadores que permitam a identificação dos doentes que irão responder à terapia, poupando os que não respondem aos efeitos adversos. Para além deste aspeto, parece promissor o impacto na clínica da utilização destes biomarcadores na monitorização da resposta durante o tratamento. Dado o reconhecido papel do sistema imunológico na resposta anti-tumoral, estas células têm sido intensamente estudadas como potenciais biomarcadores. Com este objetivo, o sangue periférico (SP), tem revelado grande interesse e importância, dada a sua fácil acessibilidade e natureza menos invasiva. A avaliação detalhada e integral da imunidade periférica, exige uma metodologia multiparamétrica como a citometria de fluxo, recorrendo à utilização de marcadores de linhagem simultaneamente com marcadores de maturação, ativação e estados funcionais. Com esta revisão bibliográfica narrativa, pretendeu-se descrever o “estado da arte” sobre o estudo por citometria de fluxo das populações celulares do sistema imunológico no SP, como potenciais biomarcadores para a terapia com ICI em tumores sólidos. Os resultados encontrados são apresentados para cada uma das populações principais e suas subpopulações, nomeadamente linfócitos T, células supressoras derivadas da linhagem mieloide (MDSCs), neutrófilos, eosinófilos, células dendríticas (DC), células natural killer (NK), monócitos, e linfócitos B. 

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Publicado
2021-01-20
Como Citar
TRIGO, Ana Catrina et al. AVALIAÇÃO DE BIOMARCADORES NO SANGUE PERIFÉRICO POR CITOMETRIA DE FLUXO COMO MEIO DE ORIENTAÇÃO PARA A IMUNOTERAPIA EM TUMORES SÓLIDOS: REVISÃO DA LITERATURA. Revista Portuguesa de Cirurgia, [S.l.], n. 49, p. 48-60, jan. 2021. ISSN 2183-1165. Disponível em: <https://revista.spcir.com/index.php/spcir/article/view/852>. Acesso em: 03 mar. 2021. doi: https://doi.org/10.34635/rpc.852.
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Artigos de Revisão